NGS Controls & Reference Materials
Infectious Disease
SHOP PRODUCTSPatient-like infectious disease drug resistance reference materials for NGS assays
Clinical genomics is emerging as a powerful tool in the detection, monitoring, and treatment of HIV. Increasingly, emergent viral strains with genotypes conferring resistance to popular combination therapies are hindering treatment efficacy. The need for highly sensitive assays to detect viral mutations implicated in drug resistance - even at very low copies - to help determine appropriate therapy is acute.
Seraseq® HIV drug resistance reference materials are full-process recombinant virus standards that contain many clinically relevant mutations.
- Non-infectious, replication-defective virus
- Clinically relevant variants in all key gene regions
- Useful for NGS and Sanger sequencing platforms
- Determine assay sensitivity and LOD with 20%, 5%, and 1% variant frequencies
- Easily customizable to any viral sequence (such as HCV) and variant frequency
Mutations Included in the Seraseq HIV-1 Drug Resistance and Tropism Reference Materials
- 49 unique mutation variants
|
Gag
|
H219Q
CAT to CAA) |
G381S
(GGC to AGC) |
|
| Protease Gene |
L24I
(TTA to ATA) |
D30N
(GAT to AAT) |
V32I
(GTA to ATA) |
M46I
(ATG to ATA) |
I47V
(ATA to CTA) |
G48V
(GGG to GTG) |
I50V
(ATT to GTT); |
I54M
(ATC to ATG) |
G73S
(GGT to GCT) |
L76V
(TTA to GTA) |
V83A
(GTC to GCC) |
I84V
(ATA to GTA) |
N88D
(AAT to GAT) |
L90M
(TTG to ATG) |
|
| RT Gene (NRTI) |
M41L
(ATG to TTG) |
K65F
(AAA to AGA) |
D67N
(GAC to AAC) |
T69S
(ACT to TCT) |
K70R
(AAA to AGA) |
L74V
(TTA to GTA) |
F77L
(TCC to CTC) |
Y115F
(TAT to TTT) |
F116Y
(TTT to TAT) |
Q151M
(CAG to ATG) |
M184V
(ATG to GTG) |
L210W
(TTG to TGG) |
T215Y
(ACC to TAC) |
K219Q
(AAA to CAA) |
|
| RT Gene (NNRTI) |
L100I
(TTA to ATA) |
K101E
(AAA to GAA) |
K103N
(AAA toAAC) |
V108I
(GTA to ATA) |
V106A
(GTA to GCA) |
Y181C
(TAT to TGT) |
Y188L
(TAT to TTA) |
G190A
(GGA to GCA) |
P22H5
(CCT to CAT) |
M230L
(ATG to CTG) |
|
Compatibility
