New

Clinical Lab Quality Control

Accukit™ Myeloid DNA IS unfragmented

Material Number AG-2023
Size 2 x 50 uL
Inventory Status Backorder: Available Soon
Unit EA

Standardized Nucleic Acid Quantification for Sequencing (SNAQ-SEQ) is a critical QC method to provide variant specific sensitivity controls for therapeutically relevant Acute Myeloid Leukemia (AML) targets in each sample. Mixtures of synthetic DNA created to mimic the sample regions of interest are added to each sample prior to NGS library preparation; they covary through the complex chemistry, flow cell detection and bioinformatics workflow, mirroring the samples native target template to provide the ideal assay run control. Internal standards (IS) bring the reference material directly into the sample, eliminating the need for external run controls for more cost-efficient reagent and flow cell utilization, while also providing direct QC of the sample.

Accukit Myeloid DNA IS are added to each sample as a single reagent addition, represent genomic regions with clinically relevant cancer driver variants, and are compatible with hybrid capture and amplicon-based library prep chemistries.

LGC is proud to be the exclusive distributor for North America, UK and Europe.

VARIANT
#
CHROM
POS
REF
ALT
Gene
Mutation-nt
Mutation-aa
SNP
1
chr9 
133748281
C
T
ABL1
c.942C>T
p.lle314
 
2
chr2 
25457241
G
A
DNMT3A
c.2646C>T
p.Arg882
 
3
chr13 
28592644
C
A
FLT3
c.2501G>T
p.Arg834Leu
 
4
chr2 
209113116
C
T
IDH1
c.391G>A
p.Gly131Ser
 
5
chr15 
90631841
C
T
IDH2
c.512G>A
p.Gly171Asp
 
6
chr9 
5073774
G
T
JAK2
c.1853G>T
p.Cys618Phe
 
7
chr12
25398288
C
T
KRAS
c.31G>T
p.Ala11Thr
 
8
chr1 
115252199
C
T
NRAS
c.441G>A
p.Lys147
 
9
chr1
115256531
T
A
NRAS
c.180A>T
p.Gly60
 
10
chr4
106164918
G
A
TET2
c.3786G>A
p.Arg1262
 
11
chr17
7577562
C
T
TP53
c.719G>A
p.Ser240Asn
INS
12
chr13 
28608248
C
C..29..T
FLT3
c.1808insA..28..T
p.Lys602_Trp603ins..Lys
 
13
chr13 
28606248
C
C..125..T
FLT3
c.1741insA..28..T
p.Gln580_Val58ins..Gln
 
14
chr5
170837550
A
AGTCT
NPM1
c.866_867insGTCT
p.Gln289Cfs*12
 
15
chr5
170837550
A
AGCAT
NPM1
c.866_867insGCAT
p.Gln289Cfs*12
 
16
chr5
170837550
A
AGCCT
NPM1
c.866_867insGCCT
p.Gln289Cfs*12
DEL
17
chr9 
5070014
TGGTGTT
T
JAK2
c.1605_1610delGGTTGT
p.Met535_Val536delinslleHis