KPL Immunoassay Reagents & Kits
Recombinant Protein Detection
SeraCare’s KPL HisDetector™ Nickel Conjugates and Western Blot Kits eliminate the time and expense of using anti-His antibodies. HisDetector products utilize nickel-NTA conjugates which bind with high affinity to poly-histidine residues. They enable simple, direct detection of His-tagged proteins without the need for primary and secondary antibodies.
Recombinant Protein Detection at Its Best
- High affinity of histidine for nickel (Ni2+) ions allows nickel-NTA (nitrilotriacetic acid) conjugates to bind directly to the His-tag
- Detection is achieved with either chemiluminescent or colorimetric substrates
- Nickel ions have six electron coordination bonds, and NTA binds to four of these bonds
- Two ligand binding sites coordinate with His-tagged proteins
Direct detection of His-tagged proteins with nickel conjugates greatly speeds assay times. Western Blot protocols take only 2.5 hours, saving several hours over traditional antibody detection methods. Assay optimization is minimal when compared to antibody methods that require optimization of multiple antibodies.